Generator
WOG
Part:BBa_J45100
Designed by: Boyuan Zhu Group: iGEM06_MIT (2006-08-09)
Wintergreen odor enzyme (BSMT1) generator
This device produces methyl salicylate in the presence of salicylic acid. Expression is regulated by R0040 (Tet repressor). Methyl salicylate smells strongly of mint (wintergreen).
Wintergreen Generating Device (WGD):
- converts salicylic acid (SA) into methyl salicylate
- production of methyl salicylate was verified both by scent and by gas chromatography: E. coli with no WGD did not produce methyl salicylate when SA was added to the medium, while E. coli with the WGD did produce methyl salicylate when SA was added to the medium.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 746
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 287
[edit]
Categories
Parameters
//classic/generator/prct
//function/odor
//function/odor
o_h | |
protein | BSMT1 |
rbs | 147 |
tag | None |